Scientists in Switzerland have developed a novel way to monitor a neuron's electrical activity by bathing it in laser light. The technique, called holographic microscopy, doesn't require the invasive electrodes or dyes typically used to measure cell activity. Researchers say the approach could be used to rapidly screen new drugs designed to protect brain cells.
Holographic microscopy shines laser light on an object and computationally reconstructs the object's form based on how the light waves are deformed. The technology is most commonly used to study materials—to search for flaws on the surfaces of lenses or microchips, for example. But scientists have recently begun to use it on living cells.
Because cells are transparent, changes to the light that passes through the cell—known as the refractive index—can be used to calculate both the cell's shape and its contents. The cell's contents are directly related to its electrical activity: when a neuron becomes electrically active, channels in the neuron's membrane open, allowing both water and ions to rush into the cell.
"The change in water content changes the refractive index, so we are able to monitor current without electrodes," says Pierre Magistretti, director of the Brain Mind Institute at the Ecole Polytechnique Fédérale de Lausanne, in Switzerland. Magistretti led the research. By using both conventional electrode recording and the holographic technique to monitor neurons grown in petri dishes, Magistretti and collaborators confirmed that holographic microscopy could accurately track electrical activity in the cell. The research was published this month in Journal of Neuroscience.
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